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dc.contributor.authorLi, Ruowei
dc.contributor.authorNarici, Marco V.
dc.contributor.authorErskine, Robert M.
dc.contributor.authorSeynnes, Olivier R.
dc.contributor.authorRittweger, Jörn
dc.contributor.authorPisot, Rado
dc.contributor.authorSimunic, Bostjan
dc.contributor.authorFlück, Martin
dc.date.accessioned2013-11-01T14:26:11Z
dc.date.available2013-11-01T14:26:11Z
dc.date.issued2013-09-08
dc.identifierSeksjon for fysisk prestasjonsevne / Department of Physical Performance
dc.identifier.citationJournal of Anatomy. 2013, 223, 525-536no_NO
dc.identifier.urihttp://hdl.handle.net/11250/170946
dc.description© 2013 The Authors. Journal of Anatomy published by John Wiley & Sons Ltd on behalf of Anatomical Society. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.no_NO
dc.description.abstractCostameres are mechano-sensory sites of focal adhesion in the sarcolemma that provide a structural anchor for myofibrils. Their turnover is regulated by integrin-associated focal adhesion kinase (FAK). We hypothesized that changes in content of costamere components (beta 1 integrin, FAK, meta-vinculin, gamma-vinculin) with increased and reduced loading of human anti-gravity muscle would: (i) relate to changes in muscle size and molecular parameters of muscle size regulation [p70S6K, myosin heavy chain (MHC)1 and MHCIIA]; (ii) correspond to adjustments in activity and expression of FAK, and its negative regulator, FRNK; and (iii) reflect the temporal response to reduced and increased loading. Unloading induced a progressive decline in thickness of human vastus lateralis muscle after 8 and 34 days of bedrest (−4% and −14%, respectively; n = 9), contrasting the increase in muscle thickness after 10 and 27 days of resistance training (+5% and +13%; n = 6). Changes in muscle thickness were correlated with changes in cross-sectional area of type I muscle fibers (r = 0.66) and beta 1 integrin content (r = 0.76) at the mid-point of altered loading. Changes in meta-vinculin and FAK-pY397 content were correlated (r = 0.85) and differed, together with the changes of beta 1 integrin, MHCI, MHCII and p70S6K, between the mid- and end-point of resistance training. By contrast, costamere protein level changes did not differ between time points of bedrest. The findings emphasize the role of FAK-regulated costamere turnover in the load-dependent addition and removal of myofibrils, and argue for two phases of muscle remodeling with resistance training, which do not manifest at the macroscopic level.no_NO
dc.language.isoengno_NO
dc.publisherJohn Wiley & Sonsno_NO
dc.subjectatrophyno_NO
dc.subjectfocal adhesion kinaseno_NO
dc.subjecthumanno_NO
dc.subjecthypertrophyno_NO
dc.subjectloadno_NO
dc.subjectmuscleno_NO
dc.subjectmyofibrilno_NO
dc.subjectvinculinno_NO
dc.titleCostamere remodeling with muscle loading and unloading in healthy young menno_NO
dc.typeJournal articleno_NO
dc.typePeer reviewedno_NO
dc.subject.nsiVDP::Mathematics and natural science: 400::Basic biosciences: 470no_NO
dc.source.journalJournal of Anatomyno_NO
dc.identifier.doi10.1111/joa.12101


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