dc.contributor.author | Li, Ruowei | |
dc.contributor.author | Narici, Marco V. | |
dc.contributor.author | Erskine, Robert M. | |
dc.contributor.author | Seynnes, Olivier R. | |
dc.contributor.author | Rittweger, Jörn | |
dc.contributor.author | Pisot, Rado | |
dc.contributor.author | Simunic, Bostjan | |
dc.contributor.author | Flück, Martin | |
dc.date.accessioned | 2013-11-01T14:26:11Z | |
dc.date.available | 2013-11-01T14:26:11Z | |
dc.date.issued | 2013-09-08 | |
dc.identifier | Seksjon for fysisk prestasjonsevne / Department of Physical Performance | |
dc.identifier.citation | Journal of Anatomy. 2013, 223, 525-536 | no_NO |
dc.identifier.uri | http://hdl.handle.net/11250/170946 | |
dc.description | © 2013 The Authors. Journal of Anatomy published by John Wiley & Sons Ltd on behalf of Anatomical Society.
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. | no_NO |
dc.description.abstract | Costameres are mechano-sensory sites of focal adhesion in the sarcolemma that provide a structural anchor for myofibrils. Their turnover is regulated by integrin-associated focal adhesion kinase (FAK). We hypothesized that changes in content of costamere components (beta 1 integrin, FAK, meta-vinculin, gamma-vinculin) with increased and reduced loading of human anti-gravity muscle would: (i) relate to changes in muscle size and molecular parameters of muscle size regulation [p70S6K, myosin heavy chain (MHC)1 and MHCIIA]; (ii) correspond to adjustments in activity and expression of FAK, and its negative regulator, FRNK; and (iii) reflect the temporal response to reduced and increased loading. Unloading induced a progressive decline in thickness of human vastus lateralis muscle after 8 and 34 days of bedrest (−4% and −14%, respectively; n = 9), contrasting the increase in muscle thickness after 10 and 27 days of resistance training (+5% and +13%; n = 6). Changes in muscle thickness were correlated with changes in cross-sectional area of type I muscle fibers (r = 0.66) and beta 1 integrin content (r = 0.76) at the mid-point of altered loading. Changes in meta-vinculin and FAK-pY397 content were correlated (r = 0.85) and differed, together with the changes of beta 1 integrin, MHCI, MHCII and p70S6K, between the mid- and end-point of resistance training. By contrast, costamere protein level changes did not differ between time points of bedrest. The findings emphasize the role of FAK-regulated costamere turnover in the load-dependent addition and removal of myofibrils, and argue for two phases of muscle remodeling with resistance training, which do not manifest at the macroscopic level. | no_NO |
dc.language.iso | eng | no_NO |
dc.publisher | John Wiley & Sons | no_NO |
dc.subject | atrophy | no_NO |
dc.subject | focal adhesion kinase | no_NO |
dc.subject | human | no_NO |
dc.subject | hypertrophy | no_NO |
dc.subject | load | no_NO |
dc.subject | muscle | no_NO |
dc.subject | myofibril | no_NO |
dc.subject | vinculin | no_NO |
dc.title | Costamere remodeling with muscle loading and unloading in healthy young men | no_NO |
dc.type | Journal article | no_NO |
dc.type | Peer reviewed | no_NO |
dc.subject.nsi | VDP::Mathematics and natural science: 400::Basic biosciences: 470 | no_NO |
dc.source.journal | Journal of Anatomy | no_NO |
dc.identifier.doi | 10.1111/joa.12101 | |