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dc.contributor.authorHjorth, Marit
dc.contributor.authorNorheim, Frode
dc.contributor.authorMeen, Astri Jeanette
dc.contributor.authorPourteymour, Shirin
dc.contributor.authorLee, Sindre
dc.contributor.authorHolen, Torgeir
dc.contributor.authorJensen, Jørgen
dc.contributor.authorBirkeland, Kåre I.
dc.contributor.authorMartinov, Vladimir Nikolkaev
dc.contributor.authorLangleite, Torgrim Mikal
dc.contributor.authorEckardt, Kristin
dc.contributor.authorDrevon, Christian A.
dc.contributor.authorKolset, Svein Olav
dc.date.accessioned2016-02-18T09:34:50Z
dc.date.available2016-02-18T09:34:50Z
dc.date.issued2015-08-19
dc.identifier.citationPhysiological Reports. 2015, 3, e12473. doi:10.14814/phy2.12473nb_NO
dc.identifier.urihttp://hdl.handle.net/11250/2379470
dc.description© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.nb_NO
dc.description.abstractRemodeling of extracellular matrix (ECM), including regulation of proteoglycans in skeletal muscle can be important for physiological adaptation to exercise. To investigate the effects of acute and long‐term exercise on the expression of ECM‐related genes and proteoglycans in particular, 26 middle‐aged, sedentary men underwent a 12 weeks supervised endurance and strength training intervention and two acute, 45 min bicycle tests (70% VO2max), one at baseline and one after 12 weeks of training. Total gene expression in biopsies from m. vastus lateralis was measured with deep mRNA sequencing. After 45 min of bicycling approximately 550 gene transcripts were >50% upregulated. Of these, 28 genes (5%) were directly related to ECM. In response to long‐term exercise of 12 weeks 289 genes exhibited enhanced expression (>50%) and 20% of them were ECM related. Further analyses of proteoglycan mRNA expression revealed that more than half of the proteoglycans expressed in muscle were significantly enhanced after 12 weeks intervention. The proteoglycan serglycin (SRGN) has not been studied in skeletal muscle and was one of few proteoglycans that showed increased expression after acute (2.2‐fold, P < 0.001) as well as long‐term exercise (1.4‐fold, P < 0.001). Cultured, primary human skeletal muscle cells expressed and secreted SRGN. When the expression of SRGN was knocked down, the expression and secretion of serpin E1 (SERPINE1) increased. In conclusion, acute and especially long‐term exercise promotes enhanced expression of several ECM components and proteoglycans. SRGN is a novel exercise‐regulated proteoglycan in skeletal muscle with a potential role in exercise adaptationnb_NO
dc.language.isoengnb_NO
dc.publisherWiley Periodicals Inc.nb_NO
dc.subjectextracellular matrixnb_NO
dc.subjectphysical activitynb_NO
dc.subjectproteoglycannb_NO
dc.subjectserglycinnb_NO
dc.subjectserpin E1nb_NO
dc.titleThe effect of acute and long‐term physical activity on extracellular matrix and serglycin in human skeletal musclenb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.subject.nsiVDP::Medical disciplines: 700nb_NO
dc.subject.nsiVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710nb_NO
dc.subject.nsiVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Human and veterinary science physiology: 718nb_NO
dc.source.journalPhysiological Reportsnb_NO
dc.description.localcodeSeksjon for fysisk prestasjonsevne / Department of Physical Performancenb_NO


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