Structure and function of human muscle fibres and muscle proteome in physically active older men
dc.contributor.author | Brocca, Lorenza | |
dc.contributor.author | McPhee, Jamie | |
dc.contributor.author | Longa, Emanuela | |
dc.contributor.author | Canepari, Monica | |
dc.contributor.author | Seynnes, Olivier R. | |
dc.contributor.author | de Vito, Giuseppe | |
dc.contributor.author | Pellegrino, Maria A. | |
dc.contributor.author | Narici, Marco | |
dc.contributor.author | Bottinelli, Roberto | |
dc.date.accessioned | 2018-09-05T07:29:03Z | |
dc.date.available | 2018-09-05T07:29:03Z | |
dc.date.created | 2017-11-15T08:50:27Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | Journal of Physiology. 2017, 595, 4823-4844. | nb_NO |
dc.identifier.issn | 0022-3751 | |
dc.identifier.uri | http://hdl.handle.net/11250/2560818 | |
dc.description | I Brage finner du siste tekst-versjon av artikkelen, og den kan inneholde ubetydelige forskjeller fra forlagets pdf-versjon. Forlagets pdf-versjon finner du på wiley.com / In Brage you'll find the final text version of the article, and it may contain insignificant differences from the journal's pdf version. The definitive version is available at wiley.com | nb_NO |
dc.description.abstract | Contradictory results have been reported on the impact of ageing on structure and functions of skeletal muscle fibres, likely to be due to a complex interplay between ageing and other phenomena such as disuse and diseases. Here we recruited healthy, physically and socially active young (YO) and elderly (EL) men in order to study ageing per se without the confounding effects of impaired physical function. In vivo analyses of quadriceps and in vitro analyses of vastus lateralis muscle biopsies were performed. In EL subjects, our results show that (i) quadriceps volume, maximum voluntary contraction isometric torque and patellar tendon force were significantly lower; (ii) muscle fibres went through significant atrophy and impairment of specific force (isometric force/cross-sectional area) and unloaded shortening velocity; (iii) myosin/actin ratio and myosin content in individual muscle fibres were not altered; (iv) the muscle proteome went through quantitative adaptations, namely an up-regulation of the content of several groups of proteins among which were myofibrillar proteins and antioxidant defence systems; (v) the muscle proteome went through qualitative adaptations, namely phosphorylation of several proteins, including myosin light chain-2 slow and troponin T and carbonylation of myosin heavy chains. The present results indicate that impairment of individual muscle fibre structure and function is a major feature of ageing per se and that qualitative adaptations of muscle proteome are likely to be more involved than quantitative adaptations in determining such a phenomenon. | nb_NO |
dc.description.abstract | Structure and function of human muscle fibres and muscle proteome in physically active older men | nb_NO |
dc.language.iso | eng | nb_NO |
dc.subject | adult | nb_NO |
dc.subject | aged | nb_NO |
dc.subject | aging/metabolism | nb_NO |
dc.subject | exercise | nb_NO |
dc.subject | human | nb_NO |
dc.subject | male | nb_NO |
dc.subject | muscle proteins/metabolism | nb_NO |
dc.subject | muscle, skeletal/metabolism | nb_NO |
dc.subject | muscle, skeletal/pathology | nb_NO |
dc.subject | muscular atrophy/metabolism | nb_NO |
dc.subject | oxidation-reduction | nb_NO |
dc.subject | phosphorylation | nb_NO |
dc.subject | protein processing, post-translational | nb_NO |
dc.subject | proteome | nb_NO |
dc.subject | young adult | nb_NO |
dc.title | Structure and function of human muscle fibres and muscle proteome in physically active older men | nb_NO |
dc.title.alternative | Structure and function of human muscle fibres and muscle proteome in physically active older men | nb_NO |
dc.type | Journal article | nb_NO |
dc.type | Peer reviewed | nb_NO |
dc.description.version | acceptedVersion | nb_NO |
dc.source.pagenumber | 4823-4844 | nb_NO |
dc.source.volume | 595 | nb_NO |
dc.source.journal | Journal of Physiology | nb_NO |
dc.source.issue | 14 | nb_NO |
dc.identifier.doi | 10.1113/JP274148 | |
dc.identifier.cristin | 1514226 | |
dc.description.localcode | Seksjon for fysisk prestasjonsevne / Department of Physical Performance | nb_NO |
cristin.unitcode | 150,31,0,0 | |
cristin.unitname | Seksjon for fysisk prestasjonsevne | |
cristin.ispublished | true | |
cristin.fulltext | postprint | |
cristin.qualitycode | 2 |
Tilhørende fil(er)
Denne innførselen finnes i følgende samling(er)
-
Artikler / Articles [2119]