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dc.contributor.authorChowdhury, Helena H.
dc.contributor.authorKreft, Marko
dc.contributor.authorJensen, Jørgen
dc.contributor.authorZorec, Robert
dc.date.accessioned2015-03-20T11:56:49Z
dc.date.available2015-03-20T11:56:49Z
dc.date.issued2014-10-02
dc.identifier.citationInternational Journal of Molecular Sciences. 2014, 15, 17827-17837nb_NO
dc.identifier.urihttp://hdl.handle.net/11250/279997
dc.descriptionThis is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.nb_NO
dc.description.abstractGlucose is an important source of energy for mammalian cells and enters the cytosol via glucose transporters. It has been thought for a long time that glucose entering the cytosol is swiftly phosphorylated in most cell types; hence the levels of free glucose are very low, beyond the detection level. However, the introduction of new fluorescence resonance energy transfer-based glucose nanosensors has made it possible to measure intracellular glucose more accurately. Here, we used the fluorescent indicator protein (FLIPglu-600µ) to monitor cytosolic glucose dynamics in mouse 3T3-L1 cells in which glucose utilization for glycogen synthesis was inhibited. The results show that cells exhibit a low resting cytosolic glucose concentration. However, in cells with inhibited glycogen synthase activation, insulin induced a robust increase in cytosolic free glucose. The insulin-induced increase in cytosolic glucose in these cells is due to an imbalance between the glucose transported into the cytosol and the use of glucose in the cytosol. In untreated cells with sensitive glycogen synthase activation, insulin stimulation did not result in a change in the cytosolic glucose level. This is the first report of dynamic measurements of cytosolic glucose levels in cells devoid of the glycogen synthesis pathway.nb_NO
dc.language.isoengnb_NO
dc.publisherMDPInb_NO
dc.subjectglucosenb_NO
dc.subjectFRETnb_NO
dc.subjectnanosensornb_NO
dc.subject3T3-L1 fibroblastnb_NO
dc.subjectinsulinnb_NO
dc.subjectglycogennb_NO
dc.titleInsulin induces an increase in cytosolic glucose levels in 3T3-L1 cells with inhibited glycogen synthase activationnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.subject.nsiVDP::Mathematics and natural science: 400::Chemistry: 440nb_NO
dc.subject.nsiVDP::Medical disciplines: 700nb_NO
dc.source.journalInternational Journal of Molecular Sciencesnb_NO
dc.description.localcodeSeksjon for fysisk prestasjonsevne / Department of Physical Performancenb_NO


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