Human skeletal muscle fibre contractile properties and proteomic profile: adaptations to 3 weeks of unilateral lower limb suspension and active recovery
Brocca, Lorenza; Longa, Emanuela; Cannavino, Jessica; Seynnes, Olivier R.; de Vito, Giuseppe; McPhee, Jamie; Narici, Marco; Pellegrino, Maria Antonietta; Bottinelli, Roberto
Journal article, Peer reviewed
Permanent lenke
http://hdl.handle.net/11250/2404174Utgivelsesdato
2015-12-15Metadata
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Sammendrag
Following disuse, muscle fibre function goes through adaptations such as a loss of specific force (PO/CSA) and an increase in unloaded shortening velocity, which could be a result of both quantitative changes (i.e. atrophy) and qualitative changes in protein pattern. The underlying mechanisms remain to be clarified. In addition, little is known about the recovery of muscle mass and strength following disuse. In the present study, we report an extensive dataset describing, in detail,the functional and protein content adaptations of skeletal muscle in response to both disuse and re-training. Eight young healthy subjects were subjected to 3 weeks of unilateral lower limb suspension (ULLS), a widely used human model of disuse skeletal muscle atrophy. Needle biopsies samples were taken from the vastus lateralis muscle Pre-ULLS, Post-ULLS and after 3 weeks of recovery during which heavy resistance training was performed. After disuse, cross-sectional area (CSA), PO/CSA and myosin concentration (MC) decreased in both type 1 and 2A skinned muscle fibres. After recovery, CSA and MC returned to levels comparable to those observed before disuse, whereas Po/CSA and unloaded shortening velocity reached a higher level. Myosin heavy chain isoform composition of muscle samples did not differ among the experimental groups. To study the mechanisms underlying such adaptations, a two-dimensional proteomic analysis was performed. ULLS induced a reduction of myofibrillar, metabolic (glycolytic and oxidative) and anti-oxidant defence system protein content. Resistance training was very effective in counteracting ULLS-induced alterations, indicating that long-term ULLS did not prevent the positive effect of exercise on human muscle.
Beskrivelse
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Utgiver
The Physiological SocietyTidsskrift
Journal of PhysiologyBeslektede innførsler
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Objectively measured sedentary time, physical activity and kidney function in people with recently diagnosed Type 2 diabetes: a prospective cohort analysis
Guo, Vivianyawei; Brage, Søren; Ekelund, Ulf; Griffin, Simon J.; Simmons, Rebecca K. (Journal article; Peer reviewed, 2015-09-08)Aim: To assess the prospective association between objectively measured physical activity and kidney function over 4 years in people with Type 2 diabetes. Methods: Individuals (120 women and 206 men) participating in the ... -
Structure and function of human muscle fibres and muscle proteome in physically active older men
Brocca, Lorenza; McPhee, Jamie; Longa, Emanuela; Canepari, Monica; Seynnes, Olivier R.; de Vito, Giuseppe; Pellegrino, Maria A.; Narici, Marco; Bottinelli, Roberto (Journal article; Peer reviewed, 2017)Contradictory results have been reported on the impact of ageing on structure and functions of skeletal muscle fibres, likely to be due to a complex interplay between ageing and other phenomena such as disuse and diseases. ... -
Higher lipid turnover and oxidation in cultured human myotubes from athletic versus sedentary young male subjects
Lund, Jenny; Helle, Siw A.; Li, Yuchuan; Løvsletten, Nils Gunnar; Stadheim, Hans Kristian; Jensen, Jørgen; Kase, Eili Tranheim; Thoresen, G. Hege; Rustan, Arild (Journal article; Peer reviewed, 2018)In this study we compared fatty acid (FA) metabolism in myotubes established from athletic and sedentary young subjects. Six healthy sedentary (maximal oxygen uptake (VO2max)≤ 46 ml/kg/min) and six healthy athletic ...