Low-input RNA-sequencing in patients with cartilage lesions, osteoarthritis, and healthy cartilage
Wang, Katherine; Esbensen, Qin Ying; Karlsen, Tommy Aleksander; Eftang, Cathrine Nørstad; Owesen, Christian; Årøen, Asbjørn; Jakobsen, Rune Bruhn
Peer reviewed, Journal article
Published version
Permanent lenke
https://hdl.handle.net/11250/2984337Utgivelsesdato
2021Metadata
Vis full innførselSamlinger
- Artikler / Articles [2119]
- Publikasjoner fra Cristin [1107]
Sammendrag
Objective
To analyze and compare cartilage samples from 3 groups of patients utilizing low-input RNA-sequencing.
Design
Cartilage biopsies were collected from patients in 3 groups (n = 48): Cartilage lesion (CL) patients had at least ICRS grade 2, osteoarthritis (OA) samples were taken from patients undergoing knee replacement, and healthy cartilage (HC) was taken from ACL-reconstruction patients without CLs. RNA was isolated using an optimized protocol. RNA samples were assessed for quality and sequenced with a low-input SmartSeq2 protocol.
Results
RNA isolation yielded 48 samples with sufficient quality for sequencing. After quality control, 13 samples in the OA group, 9 in the HC group, and 9 in the CL group were included in the analysis. There was a high degree of co-clustering between the HC and CL groups with only 6 genes significantly up- or downregulated. OA and the combined HC/CL group clustered significantly separate from each other, yielding 659 significantly upregulated and 1,369 downregulated genes. GO-term analysis revealed that genes matched to cartilage and connective tissue development terms.
Conclusion
The gene expression profiles from the 3 groups suggest that there are no major differences in gene expression between cartilage from knees with a cartilage injury and knees without an apparent cartilage injury. OA cartilage, as expected, showed markedly different gene expression from the other 2 groups. The gene expression profiles resulting from this low-input RNA-sequencing study offer opportunities to discover new pathways not previously recognized that may be explored in future studies.
Beskrivelse
This article is distributed under the terms of the Creative Commons Attribution 4.0 License (https://creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).
Tidsskrift
CartilageOpphavsrett
© The Author(s) 2021Beslektede innførsler
Viser innførsler beslektet ved tittel, forfatter og emneord.
-
Development of a pilot cartilage surgery register
Engen, Cathrine Nørstad; Årøen, Asbjørn; Engebretsen, Lars (Peer reviewed; Journal article, 2017)Background: Norway has no prospective surveillance system to monitor the outcome of knee cartilage surgery. In 2004 the Norwegian Registry of Knee Ligament (NKLR) was successfully established, and has yielded useful ... -
Is T2 mapping reliable in evaluation of native and repair cartilage tissue of the knee?
Banitalebi, Hasan; Owesen, Christian; Årøen, Asbjørn; Tran, Hang Thi; Myklebust, Tor Åge; Randsborg, Per-Henrik (Peer reviewed; Journal article, 2021)Purpose: To evaluate the effect of imaging plane and experience of observers on the reliability of T2 mapping of native and repair cartilage tissue of the knee. Methods: Fifteen consecutive patients from two randomised ... -
No degeneration found in focal cartilage defects evaluated with dGEMRIC at 12-year follow-up
Engen, Cathrine Nørstad; Løken, Sverre; Årøen, Asbjørn; Ho, Charles; Engebretsen, Lars (Journal article; Peer reviewed, 2016-11-24)Background and purpose: The natural history of focal cartilage defects (FCDs) is still unresolved, as is the long-term cartilage quality after cartilage surgery. It has been suggested that delayed gadolinium-enhanced ...