Role of glycogen content on glucose uptake and glycogen synthase in skeletal muscles: The effect of contraction and insulin
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The present thesis is based on 4 papers which have investigated contraction- and insulin-mediated regulations of glucose uptake and GS activation in skeletal muscles with different glycogen contents. The level of glycogen content in skeletal muscle has a strong effect on the regulation of glucose metabolism. In the present thesis, we have shown that low glycogen content strongly increased glucose uptake in epitrochlearis muscle either under insulin and contraction stimulation alone or in combination. GLUT4 content was increased in epitrochlearis muscle with low glycogen content which can contribute the increased glucose uptake. Although insulin-stimulated PKB phosphorylation and contraction-stimulated AMPK activation were highest in muscle with low glycogen content, AS160/TBC1D1 phosphorylation was not elevated correspondingly, suggesting that these key signalling molecules do not determine the rate of glucose uptake. Furthermore, low glycogen content prevents the additivity of glucose uptake when insulin and contraction were combined.Paper I: Tatt ut av filen i Brage p.g.a. copyright-restriksjoner. / Not in the file in Brage because of copyright issues.Paper II: Tatt ut av filen i Brage p.g.a. copyright-restriksjoner. / Not in the file in Brage because of copyright issues.Paper III: Tatt ut av filen i Brage p.g.a. copyright-restriksjoner. / Not in the file in Brage because of copyright issues.Paper IV: Lai YC, Lin FC & Jensen J (2009). Glycogen content regulates insulin- but not contraction-mediated glycogen synthase activation in the rat slow-twitch soleus muscles. Acta Physiol. 197(2): 139-150.
Avhandling (doktorgrad) – Norges idrettshøgskole, 2010.